ArticleThe EMBO journal1999
The disulfide-bonded loop of chromogranin B mediates membrane binding and directs sorting from the trans-Golgi network to secretory granules.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 119 citations in OpenAlex.
- Cell-specific secretory granule sorting mechanisms: the role of MAGEL2 and retromer in hypothalamic regulated secretion.Frontiers in cell and developmental biology · 2023Review
- Trafficking of hormones and trophic factors to secretory and extracellular vesicles: a historical perspective and new hypothesis.Extracellular vesicles and circulating nucleic acids · 2023Article
- Liquid-liquid phase separation facilitates the biogenesis of secretory storage granules.The Journal of cell biology · 2022Article
- Small disulfide loops in peptide hormones mediate self-aggregation and secretory granule sorting.Life science alliance · 2022Article
- Inside the Insulin Secretory Granule.Metabolites · 2021Review
- Chromogranin A, the major lumenal protein in chromaffin granules, controls fusion pore expansion.The Journal of general physiology · 2019Article
- Amyloid and intracellular accumulation of BRINeurobiology of aging · 2017Article
- Carboxypeptidase E and Secretogranin III Coordinately Facilitate Efficient Sorting of Proopiomelanocortin to the Regulated Secretory Pathway in AtT20 Cells.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Stability of proICA512/IA-2 and its targeting to insulin secretory granules require β4-sheet-mediated dimerization of its ectodomain in the endoplasmic reticulum.Molecular and cellular biology · 2015Article
- The functional role of chromogranins in exocytosis.Journal of molecular neuroscience : MN · 2012Article
- C-terminal domain of chromogranin B regulates intracellular calcium signaling.The Journal of biological chemistry · 2011Article
- The extended granin family: structure, function, and biomedical implications.Endocrine reviews · 2011Review
- Signal transduction of pregnenolone sulfate in insulinoma cells: activation of Egr-1 expression involving TRPM3, voltage-gated calcium channels, ERK, and ternary complex factors.The Journal of biological chemistry · 2011Article
- Chromogranins A and B as regulators of vesicle cargo and exocytosis.Cellular and molecular neurobiology · 2010Review
- AlphaS1-casein, which is essential for efficient ER-to-Golgi casein transport, is also present in a tightly membrane-associated form.BMC cell biology · 2010Article
- Regulation of the hypothalamic thyrotropin releasing hormone (TRH) neuron by neuronal and peripheral inputs.Frontiers in neuroendocrinology · 2010Review
- Chromogranin B gene ablation reduces the catecholamine cargo and decelerates exocytosis in chromaffin secretory vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- A large form of secretogranin III functions as a sorting receptor for chromogranin A aggregates in PC12 cells.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Beta cell chromogranin B is partially segregated in distinct granules and can be released separately from insulin in response to stimulation.Diabetologia · 2008Article
- Sorting of the neuroendocrine secretory protein Secretogranin II into the regulated secretory pathway: role of N- and C-terminal alpha-helical domains.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The disulfide-bonded loop of chromogranin B (CgB), a regulated secretory protein with widespread distribution in neuroendocrine cells, is known to be essential for the sorting of CgB from the trans-Golgi network (TGN) to immature secretory granules. Here we show that this loop, when fused to the constitutively secreted protein alpha1-antitrypsin (AT), is sufficient to direct the fusion protein to secretory granules. Importantly, the sorting efficiency of the AT reporter protein bearing two loops (E2/3-AT-E2/3) is much higher compared with that of AT with a single disulfide-bonded loop. In contrast to endogenous CgB, E2/3-AT-E2/3 does not undergo Ca2+/pH-dependent aggregation in the TGN. Furthermore, the disulfide-bonded loop of CgB mediates membrane binding in the TGN and does so with 5-fold higher efficiency if two loops are present on the reporter protein. The latter finding supports the concept that under physiological conditions, aggregates of CgB are the sorted units of cargo which have multiple loops on their surface leading to high membrane binding and sorting efficiency of CgB in the TGN.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.