ArticleThe EMBO journal1999
In vivo expression of the nucleolar group I intron-encoded I-dirI homing endonuclease involves the removal of a spliceosomal intron.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
- Telomere-to-telomere characterization of rDNA chromosome in the myxomycete Didymium iridis.BMC molecular and cell biology · 2026Article
- Mobile group I introns at nuclear rDNA position L2066 harbor sense and antisense homing endonuclease genes intervened by spliceosomal introns.Mobile DNA · 2022Article
- Article
- A Phylogenetic Approach to Structural Variation in Organization of Nuclear Group I Introns and Their Ribozymes.Non-coding RNA · 2021Article
- Lariat capping as a tool to manipulate the 5' end of individual yeast mRNA species in vivo.RNA (New York, N.Y.) · 2017Article
- Accumulation of Stable Full-Length Circular Group I Intron RNAs during Heat-Shock.Molecules (Basel, Switzerland) · 2016Article
- Review
- Circular RNAs: diversity of form and function.RNA (New York, N.Y.) · 2014Review
- Speciation of a group I intron into a lariat capping ribozyme.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Molecular characterization of a new member of the lariat capping twin-ribozyme introns.Mobile DNA · 2014Article
- Nuclear group I introns in self-splicing and beyond.Mobile DNA · 2013Article
- A conformational switch in the DiGIR1 ribozyme involved in release and folding of the downstream I-DirI mRNA.RNA (New York, N.Y.) · 2009Article
- Molecular modelling of the GIR1 branching ribozyme gives new insight into evolution of structurally related ribozymes.The EMBO journal · 2008Article
- The recent transfer of a homing endonuclease gene.Nucleic acids research · 2005Article
- Site-specific reverse splicing of a HEG-containing group I intron in ribosomal RNA.Nucleic acids research · 2005Article
- The ability to form full-length intron RNA circles is a general property of nuclear group I introns.RNA (New York, N.Y.) · 2003Article
- Article
- Flanking sequences with an essential role in hydrolysis of a self-cleaving group I-like ribozyme.Nucleic acids research · 2000Article
- Expression of the Naegleria intron endonuclease is dependent on a functional group I self-cleaving ribozyme.RNA (New York, N.Y.) · 2000Article
- Functional alpha-fragment of beta-galactosidase can be expressed from the mobile group I intron PpLSU3 embedded in yeast pre-ribosomal RNA derived from the chromosomal rDNA locus.Nucleic acids research · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Didymium iridis DiSSU1 intron is located in the nuclear SSU rDNA and has an unusual twin-ribozyme organization. One of the ribozymes (DiGIR2) catalyses intron excision and exon ligation. The other ribozyme (DiGIR1), which along with the endonuclease-encoding I-DirI open reading frame (ORF) is inserted in DiGIR2, carries out hydrolysis at internal processing sites (IPS1 and IPS2) located at its 3' end. Examination of the in vivo expression of DiSSU1 shows that after excision, DiSSU1 is matured further into the I-DirI mRNA by internal DiGIR1-catalysed cleavage upstream of the ORF 5' end, as well as truncation and polyadenylation downstream of the ORF 3' end. A spliceosomal intron, the first to be reported within a group I intron and the rDNA, is removed before the I-DirI mRNA associates with the polysomes. Taken together, our results imply that DiSSU1 uses a unique combination of intron-supplied ribozyme activity and adaptation to the general RNA polymerase II pathway of mRNA expression to allow a protein to be produced from the RNA polymerase I-transcribed rDNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.